dna binding domain meaning in English
dna结合性结构域
dna结合域,dna结合区
结合域
Examples
- 2 . a bhlh - type gene osbhlhl was obtained from the rice cdna library by using the rapid cloning pcr . the predicted osbhlhl protein had a nucleus location signal , a putative dna binding domain contained a bhlh - bzip domain
2利用pcr筛库法,从水稻cdna文库中克隆到一个bhlh类型的基因,命名为osbhlh1 ,它的推测蛋白结构中含有一个细胞核定位信号和一个可能的与dna结合bhlh - zip结构域。 - The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga . - galactosidase assay indicated that got did not have the property of self - activation . after pgbkt7 - ga was transformed to yeast pj69 - 4a , we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通过pcr扩增得到gpa1基因并将其克隆到双杂交dna结合域载体pgbkt7中,得到的重组质粒命名为pgbkt7 - g , ?半乳糖苷酶活性鉴定表明g不具有自激活特性,将其转化到酵母菌pj69 - 4a中,再以此为受体菌转化拟南芥绿色营养组织cdna文库质粒。